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Human Ubiquitin


Recombinant Human Ubiquitin (genbank accession number 229532), with a C-terminal extension (HS UBI-SHHHHHH), is expressed in E.coli using our cell growth medium. The protein is purified by non-denaturing methods to homogeneity as determined by SDS-PAGE. Purified protein is lyophilized under conditions preventing deleterious pH changes and packaged under vacuum. Performance is ensured for each batch by nuclear magnetic resonance (NMR) testing.

(Storage: Lyophilized protein in unopened containers may be stored for extended periods at -20°C. Once solubilized, the protein should be stored at 4°C and aseptic conditions should be maintained.)

1H15N-HSQC NMR spectrum of uniformly 15N,13C,2H-labeled Ubiquitin. Sample concentration was 0.7 mM in 50 mM phosphate buffer in 90% H2O/10% D2O at pH=6 with 1mM NaN3. NMR measurements were performed at 30ºC on a 500 MHz system. The spectrum was recorded with 128 msec acquisition time in the proton dimension and 75 msec acquisition time in the nitrogen dimension. All characteristic cross-peaks were observed and chemical shifts are in excellent agreement with published literature data (Wang et al., “Journal of Biomolecular NMR.” 1995, (376-382).

Human Ubiquitin Alphabetic Listing
Catalog Number Product Name Enrichment

Quantity

Price

 

 
 
His-Ubiquitin -13C
>98% 13C
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His-Ubiquitin -13C,15N
>98% 13C, >98% 15N
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His-Ubiquitin -13C,2H,15N
>98% 2H, >98% 13C, >98% 15N
5 mg Request Quote  
 
His-Ubiquitin -15N
>98% 15N
5 mg Request Quote  
 
His-Ubiquitin -2H, 13C
> 98% 2H, > 98% 13C
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His-Ubiquitin -2H, 15N
>98% 2H, >98% 15N
5 mg Request Quote  
 
Ubiquitin
10 mg $360     Add to Cart  
 
Ubiquitin-13C
>98% 13C
1 mg $890     Add to Cart  
5 mg $2,090     Add to Cart  
 
Ubiquitin-13C,15N
99%13C, 98%15N
1 mg $1,700     Add to Cart  
5 mg $3,800     Add to Cart  
 
Ubiquitin-15N
98% 15N
5 mg $2,500     Add to Cart  
 
Ubiquitin-2D, 15N
>98%15N, >97% 2D
10 mg $3,300     Add to Cart  
 
Ubiquitin-2H,13C,15N
>98%15N, >97%D, >97%13C
5 mg $4,990     Add to Cart  
 
Ubiquitin-2H
97% 2H
10 mg $1,850     Add to Cart  

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