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Parent Category: Biochemicals & Reagents
Cross-linking is the process of chemically joining two or more molecules by a covalent bond. Cross-linking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. Because of the availability of several chemical groups in proteins and peptides that may be targets for reactions, proteins and peptides are readily conjugated and otherwise studied using cross-linking methods. Cross-linkers also are commonly used to modify nucleic acids, drugs and solid surfaces. Cross-linking reagents have been used to assist in determination of near-neighbor relationships, three-dimensional structures of proteins, solid-phase immobilization, hapten-carrier protein conjugation and molecular associations in cell membranes. They also are useful for preparing antibody-enzyme conjugates, immunotoxins and other labeled protein reagents. Cross-linkers are selected on the basis of their chemical reactivities (i.e., specificity for particular functional groups) and compatibility of the reaction with the application. The best cross-linker to use for a specific application must be determined empirically.
Cross Linking Reagents
Cross-linking is the process of chemically joining two or more molecules by a covalent bond. Cross-linking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. Because of the availability of several chemical groups in proteins and peptides that may be targets for reactions, proteins and peptides are readily conjugated and otherwise studied using cross-linking methods. Cross-linkers also are commonly used to modify nucleic acids, drugs and solid surfaces. Cross-linking reagents have been used to assist in determination of near-neighbor relationships, three-dimensional structures of proteins, solid-phase immobilization, hapten-carrier protein conjugation and molecular associations in cell membranes. They also are useful for preparing antibody-enzyme conjugates, immunotoxins and other labeled protein reagents. Cross-linkers are selected on the basis of their chemical reactivities (i.e., specificity for particular functional groups) and compatibility of the reaction with the application. The best cross-linker to use for a specific application must be determined empirically.