Product: | N-(5-Amino-1-carboxypentyl)iminodiacetic Acid Tri-t-butyl Ester | ||||||||||||
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Catalog Number: | 6425 | ||||||||||||
CAS Number: | 205379-08-4 | ||||||||||||
Pricing: |
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Formula: | C22H42N2O6 | ||||||||||||
Molecular Weight: | 430.58 | ||||||||||||
Structure: | |||||||||||||
Appearance: | Yellow oil | ||||||||||||
Category: | Chelating Agents, Ligands | ||||||||||||
Stability: | Stable under recommended storage conditions | ||||||||||||
Storage: |
Store in a well closed container. Store at -20°C |
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Literature References: |
Hochuli, E., et al.: J. of Chromatography, 411, 177 (1987) |
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MSDS: | |||||||||||||
Description: |
A nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene products expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid. Also soluble in DMSO:DMF 1:1 and DMSO:CH2Cl2 1:2 |